We implemented the novel Women’s Empowerment in Fisheries Index (WEFI) (adapted from the Women’s Empowerment in Agriculture Index), among men and women fisheries value chain actors at Zambia’s Lake Bangweulu (N = 397). We found significant gender disparities favoring males across key indicators. Men were significantly more likely to report large decision-making input into fishing, processing, transporting, and selling fish, as well as sole ownership of important productive assets such as fishing and processing equipment, canoes, and mobile phones. Women were significantly more likely to report non-completion of any years of school and being ‘‘not at all comfortable” speaking in public on decisions affecting their fishing community, on decisions related to fishery governance, and to protest illegal/un- sustainable fishing practices. Women were also significantly more likely to report that – in the past four weeks – there was no food to eat in their dwelling due to lack of resources to get food, they/another household member had gone to sleep at night hungry because there was not enough food, and they/an-other household member had gone a whole day and night without eating because there was not enough food.